Non-radioactive detection of oligonucleotide probes by photochemical amplification of dioxetanes.
We describe a new method of non-radioactive labelling and detection of oligonucleotide probes. The approach is based on a simple chemical principle. Oligonucleotides labelled with methylene blue (a photosensitizer) are hybridized on a membrane to immobilized DNA target sequences. After hybridization...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1995
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307440/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8524657/ |
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