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Non-radioactive detection of oligonucleotide probes by photochemical amplification of dioxetanes.

We describe a new method of non-radioactive labelling and detection of oligonucleotide probes. The approach is based on a simple chemical principle. Oligonucleotides labelled with methylene blue (a photosensitizer) are hybridized on a membrane to immobilized DNA target sequences. After hybridization...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Schubert, F, Knaf, A, Möller, U, Cech, D
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1995
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307440/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8524657/
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