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Non-radioactive detection of oligonucleotide probes by photochemical amplification of dioxetanes.
We describe a new method of non-radioactive labelling and detection of oligonucleotide probes. The approach is based on a simple chemical principle. Oligonucleotides labelled with methylene blue (a photosensitizer) are hybridized on a membrane to immobilized DNA target sequences. After hybridization...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1995
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307440/ https://ncbi.nlm.nih.gov/pubmed/8524657 |
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