Reconstitution of human RNA interference in budding yeast

Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...

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Bibliografiska uppgifter
Huvudupphovsmän: Suk, Kyoungho, Choi, Jihye, Suzuki, Yo, Ozturk, Sedide B., Mellor, Joseph C., Wong, Koon Ho, MacKay, Joanna L., Gregory, Richard I., Roth, Frederick P.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2011
Ämnen:
Methods Online
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://ncbi.nlm.nih.gov/pubmed/21252293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1321
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