Reconstitution of human RNA interference in budding yeast

Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...

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Autori principali: Suk, Kyoungho, Choi, Jihye, Suzuki, Yo, Ozturk, Sedide B., Mellor, Joseph C., Wong, Koon Ho, MacKay, Joanna L., Gregory, Richard I., Roth, Frederick P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2011
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://ncbi.nlm.nih.gov/pubmed/21252293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1321
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