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Reconstitution of human RNA interference in budding yeast

Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...

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Hlavní autoři: Suk, Kyoungho, Choi, Jihye, Suzuki, Yo, Ozturk, Sedide B., Mellor, Joseph C., Wong, Koon Ho, MacKay, Joanna L., Gregory, Richard I., Roth, Frederick P.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2011
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://ncbi.nlm.nih.gov/pubmed/21252293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1321
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