Reconstitution of human RNA interference in budding yeast

Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...

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Detalhes bibliográficos
Main Authors: Suk, Kyoungho, Choi, Jihye, Suzuki, Yo, Ozturk, Sedide B., Mellor, Joseph C., Wong, Koon Ho, MacKay, Joanna L., Gregory, Richard I., Roth, Frederick P.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074155/
https://ncbi.nlm.nih.gov/pubmed/21252293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1321
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