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Design, construction, and validation of a modular library of sequence diversity standards for PCR
Methods to measure the sequence diversity of PCR-amplified DNA lack standards for use as assay calibrators and controls. Here, we present a general and economical method for developing customizable DNA standards of known sequence diversity. Standards ranging from 1 to 25,000 sequences were generated...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3073996/ https://ncbi.nlm.nih.gov/pubmed/21111699 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.11.035 |
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