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ELECTROPHORETIC MOBILITY SHIFT ASSAY OF ZINC FINGER PROTEINS: COMPETITION FOR ZN(2+) BOUND TO SP1 IN PROTOCOLS INCLUDING EDTA
The electrophoretic mobility shift assay (EMSA) offers a principal method to detect specific DNA·protein interactions. As commonly conducted, the reaction and electrophoresis running buffers contain large concentrations of EDTA. EDTA has large affinity for Zn(2+) and readily competes with zinc-finge...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3073834/ https://ncbi.nlm.nih.gov/pubmed/21396899 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jinorgbio.2010.08.012 |
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