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ELECTROPHORETIC MOBILITY SHIFT ASSAY OF ZINC FINGER PROTEINS: COMPETITION FOR ZN(2+) BOUND TO SP1 IN PROTOCOLS INCLUDING EDTA

The electrophoretic mobility shift assay (EMSA) offers a principal method to detect specific DNA·protein interactions. As commonly conducted, the reaction and electrophoresis running buffers contain large concentrations of EDTA. EDTA has large affinity for Zn(2+) and readily competes with zinc-finge...

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Sonraí Bibleagrafaíochta
Main Authors: Kothinti, Rajendra, Tabatabai, Niloofar M., Petering, David H.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2010
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3073834/
https://ncbi.nlm.nih.gov/pubmed/21396899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jinorgbio.2010.08.012
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