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Fine-mapping of shotgun template-libraries; an efficient strategy for the systematic sequencing of genomic DNA.
To test the effectiveness of ordering shotgun DNA-templates prior to sequence analysis, the 450 kb left arm of yeast chromosome XII was randomly subcloned into a phagemid vector. Clones were ordered by hybridisation to an average map density of one new insert every 125 bp and are currently used for...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1995
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307300/ https://ncbi.nlm.nih.gov/pubmed/7479026 |
| タグ: |
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