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Fine-mapping of shotgun template-libraries; an efficient strategy for the systematic sequencing of genomic DNA.

To test the effectiveness of ordering shotgun DNA-templates prior to sequence analysis, the 450 kb left arm of yeast chromosome XII was randomly subcloned into a phagemid vector. Clones were ordered by hybridisation to an average map density of one new insert every 125 bp and are currently used for...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Scholler, P, Karger, A E, Meier-Ewert, S, Lehrach, H, Delius, H, Hoheisel, J D
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1995
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307300/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7479026/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.19.3842
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