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Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.
We have measured steady-state kinetics of the N6-adenine methyltransferase Dam Mtase using as substrates non-selfcomplementary tetradecamer duplexs (d[GCCGGATCTAGACG]-d[CGTCTAGATCC-GGC]) containing the hemimethylated GATC target sequence in one or the other strand and modifications in the GATC targe...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1995
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307261/ https://ncbi.nlm.nih.gov/pubmed/7478992 |
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