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Preparation of the FhuA (TonA) receptor protein from cell envelopes of an overproducing strain of Escherichia coli K-12.

A rapid and simple method for purification of the FhuA receptor protein from cell envelopes of a FhuA-overproducing strain of Escherichia coli K-12 was developed. The overproduction of FhuA was programmed by the thermoamplifiable plasmid pHK232, which carried the fhuACD genes of pLC19-19 of the Clar...

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Detalhes bibliográficos
Main Authors: Hoffmann, H, Fischer, E, Kraut, H, Braun, V
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC214619/
https://ncbi.nlm.nih.gov/pubmed/3009396
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