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Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.

We have measured steady-state kinetics of the N6-adenine methyltransferase Dam Mtase using as substrates non-selfcomplementary tetradecamer duplexs (d[GCCGGATCTAGACG]-d[CGTCTAGATCC-GGC]) containing the hemimethylated GATC target sequence in one or the other strand and modifications in the GATC targe...

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Detalhes bibliográficos
Main Authors: Marzabal, S, DuBois, S, Thielking, V, Cano, A, Eritja, R, Guschlbauer, W
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC307261/
https://ncbi.nlm.nih.gov/pubmed/7478992
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