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Combined Use of Residual Dipolar Couplings and Solution X-ray Scattering to Rapidly Probe Rigid Body Conformational Transitions in a Non-Phosphorylatable Active Site Mutant of the 128 kDa Enzyme I Dimer

The first component of the bacterial phosphotransferase system, enzyme I (EI), is a multidomain 128 kDa dimer that undergoes large rigid body conformational transitions during the course of its catalytic cycle. Here we investigate the solution structure of a non-phosphorylatable active site mutant i...

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Main Authors: Takayama, Yuki, Schwieters, Charles D., Grishaev, Alexander, Ghirlando, Rodolfo, Clore, G. Marius
格式: Artigo
語言:Inglês
出版: 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3071875/
https://ncbi.nlm.nih.gov/pubmed/21162528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja109866w
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