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Solution Structure of the 128 kDa Enzyme I Dimer from Escherichia coli and its 146 kDa Complex With HPr Using Residual Dipolar Couplings and Small and Wide Angle X-Ray Scattering

The solution structures of free Enzyme I (EI, ∼128 kDa, 575×2 residues), the first enzyme in the bacterial phosphotransferse system and its complex with HPr (∼146 kDa) have been solved using novel methodology that makes use of prior structural knowledge (namely, the structures of the dimeric EIC dom...

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Bibliographische Detailangaben
Hauptverfasser: Schwieters, Charles D., Suh, Jeong-Yong, Grishaev, Alexander, Ghirlando, Rodolfo, Takayama, Yuki, Clore, G. Marius
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2955445/
https://ncbi.nlm.nih.gov/pubmed/20731394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja105485b
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