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Investigation of the 'n-1' impurity in phosphorothioate oligodeoxynucleotides synthesized by the solid-phase beta-cyanoethyl phosphoramidite method using stepwise sulfurization.

Electrospray ionization mass spectrometry (ESI-MS) of reversed-phase HPLC-purified phosphorothioate oligodeoxynucleotides (S-ODNs), and the single-('n - 1') and double-nucleotide deletion ('n - 2') impurities subsequently isolated from them by preparative polyacrylamide gel electrophoresis (PAGE), h...

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Udgivet i:Nucleic Acids Res
Principais autores: Fearon, K L, Stults, J T, Bergot, B J, Christensen, L M, Raible, A M
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1995
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307101/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7651837/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.14.2754
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