Synthesis, deprotection, analysis and purification of RNA and ribozymes.

Improvements in the synthesis, deprotection and purification of oligoribonucleotides are described. These advances allow for reduced synthesis and deprotection times, while improving product yield. Coupling times are reduced by half using 5-ethylthio-1H-tetrazole (S-ethyltetrazole) as the activator....

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Hlavní autoři: Wincott, F, DiRenzo, A, Shaffer, C, Grimm, S, Tracz, D, Workman, C, Sweedler, D, Gonzalez, C, Scaringe, S, Usman, N
Médium: Artigo
Jazyk:Inglês
Vydáno: 1995
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC307092/
https://ncbi.nlm.nih.gov/pubmed/7544462
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