Dynamics of the ssDNA Recognition by the RepA Hexameric Helicase of Plasmid RSF1010: Analyses Using Fluorescence Stopped-Flow Intensity and Anisotropy Methods

The kinetic mechanism of the single-stranded DNA (ssDNA) recognition by the RepA hexameric replicative helicase of the plasmid RSF1010 and the nature of formed intermediates, in the presence of the ATP nonhydrolyzable analog, β,γ-imidoadenosine-5′-triphosphate (AMP-PNP), have been examined, using th...

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Hauptverfasser: Andreeva, Iraida E., Szymanski, Michal R., Jezewska, Maria J., Galletto, Roberto, Bujalowski, Wlodzimierz
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3070410/
https://ncbi.nlm.nih.gov/pubmed/19289128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.03.027
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