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EPR Spin Trapping of an Oxalate-Derived Free Radical in the Oxalate Decarboxylase Reaction
EPR spin trapping experiments on bacterial oxalate decarboxylase from Bacillus subtilis under turn-over conditions are described. The use of doubly (13)C-labeled oxalate leads to a characteristic splitting of the observed radical adducts using the spin trap N-tert-butyl-α-phenylnitrone linking them...
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| Glavni autori: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3070241/ https://ncbi.nlm.nih.gov/pubmed/21277974 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2011.01.023 |
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