EPR Spin Trapping of an Oxalate-Derived Free Radical in the Oxalate Decarboxylase Reaction

EPR spin trapping experiments on bacterial oxalate decarboxylase from Bacillus subtilis under turn-over conditions are described. The use of doubly (13)C-labeled oxalate leads to a characteristic splitting of the observed radical adducts using the spin trap N-tert-butyl-α-phenylnitrone linking them...

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Bibliographic Details
Main Authors: Imaram, Witcha, Saylor, Benjamin T., Centonze, Christopher P., Richards, Nigel G. J., Angerhofer, Alexander
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3070241/
https://ncbi.nlm.nih.gov/pubmed/21277974
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2011.01.023
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