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Enzymatic synthesis of RNA oligonucleotides.
Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, togeth...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1987
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306133/ https://ncbi.nlm.nih.gov/pubmed/3628005 |
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