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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis*

An enzyme, which is probably identical with polynucleotide phosphorylase, was prepared from Escherichiacoli B. In the presence of Mn(2+) it catalyzes the addition of one (and to a slight extent more) residue of deoxyribonucleotide residue from the diphosphate to an oligodeoxyribonucleotide primer. T...

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Detalhes bibliográficos
Main Authors: Gillam, Shirley, Waterman, Kimberley, Doel, Michael, Smith, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC343445/
https://ncbi.nlm.nih.gov/pubmed/4615302
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