Enzymatic synthesis of RNA oligonucleotides.
Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, togeth...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1987
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306133/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3628005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.16.6705 |
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