Expression, purification and preliminary X-ray crystallographic analysis of cyanobacterial biliverdin reductase

Biliverdin reductase (BVR) catalyzes the conversion of biliverdin IX α to bilirubin IX α with concomitant oxidation of an NADH or NADPH cofactor. This enzyme also binds DNA and enhances the transcription of specific genes. Recombinant cyanobacterial BVR was overexpressed in Escherichia coli, purifie...

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Bibliographic Details
Main Authors: Watanabe, Aya, Hirata, Kunio, Hagiwara, Yoshinori, Yutani, Yuko, Sugishima, Masakazu, Yamamoto, Masaki, Fukuyama, Keiichi, Wada, Kei
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2011
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3053154/
https://ncbi.nlm.nih.gov/pubmed/21393834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110053431
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