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High Throughput Screen for Escherichia coli Heat Shock Protein 70 (Hsp70/DnaK): ATPase Assay in Low Volume By Exploiting Energy Transfer

Members of the heat shock protein 70 (Hsp70) family of molecular chaperones are emerging as potential therapeutic targets. Their ATPase activity has classically been measured using colorimetric phosphate-detection reagents, such as quinaldine red (QR). While such assays are suitable for 96-well plat...

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Detalhes bibliográficos
Main Authors: Miyata, Yoshinari, Chang, Lyra, Bainor, Anthony, McQuade, Thomas J., Walczak, Christopher P., Zhang, Yaru, Larsen, Martha J., Kirchhoff, Paul, Gestwicki, Jason E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3052282/
https://ncbi.nlm.nih.gov/pubmed/20926844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057110380571
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