A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.
We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
1987
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305199/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2440031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.14.4831 |
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