A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Gunning, P, Leavitt, J, Muscat, G, Ng, S Y, Kedes, L
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1987
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305199/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2440031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.14.4831
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