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Removing Noise From Pyrosequenced Amplicons

BACKGROUND: In many environmental genomics applications a homologous region of DNA from a diverse sample is first amplified by PCR and then sequenced. The next generation sequencing technology, 454 pyrosequencing, has allowed much larger read numbers from PCR amplicons than ever before. This has rev...

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Bibliografiske detaljer
Main Authors: Quince, Christopher, Lanzen, Anders, Davenport, Russell J, Turnbaugh, Peter J
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3045300/
https://ncbi.nlm.nih.gov/pubmed/21276213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-12-38
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