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Potential of pmoA Amplicon Pyrosequencing for Methanotroph Diversity Studies

We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Majo...

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Bibliografiske detaljer
Main Authors: Lüke, Claudia, Frenzel, Peter
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3165393/
https://ncbi.nlm.nih.gov/pubmed/21764977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05355-11
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