Designing substrate specificity by protein engineering of electrostatic interactions.

Protein engineering of electrostatic interactions between charged substrates and complementary charged amino acids, at two different sites in the substrate binding cleft of the protease subtilisin BPN', increases kcat/Km toward complementary charged substrates (up to 1900 times) and decreases k...

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Hlavní autoři: Wells, J A, Powers, D B, Bott, R R, Graycar, T P, Estell, D A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1987
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC304398/
https://ncbi.nlm.nih.gov/pubmed/3547407
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