Designing substrate specificity by protein engineering of electrostatic interactions.

Protein engineering of electrostatic interactions between charged substrates and complementary charged amino acids, at two different sites in the substrate binding cleft of the protease subtilisin BPN', increases kcat/Km toward complementary charged substrates (up to 1900 times) and decreases k...

Full description

Saved in:
Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Wells, J A, Powers, D B, Bott, R R, Graycar, T P, Estell, D A
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC304398/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3547407/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.5.1219
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items