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IN VITRO Hybridization and Separation of Hybrids of Human Adenylosuccinate Lyase from Wild Type and Disease-Associated Mutant Enzymes

Human adenylosuccinate lyase (ASL) deficiency is an inherited metabolic disease in which the majority of the patients are compound heterozygotes for the mutations that occur in the ASL gene. Starting with purified wild type and single mutant human ASL, we generated in vitro hybrids which mimic compo...

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Detalhes bibliográficos
Main Authors: De Zoysa Ariyananda, Lushanti, Antonopoulos, Christina, Currier, Jenna, Colman, Roberta F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3043148/
https://ncbi.nlm.nih.gov/pubmed/21210713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101734q
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