Fluorescence Lifetime Imaging Reveals that the Environment of the ATP Binding Site of Myosin in Muscle Senses Force

Fluorescence lifetime imaging microscopy is used to demonstrate that different loads applied to a muscle fiber change the microenvironment of the nucleotide binding pocket of myosin. Permeabilized skeletal muscle fibers in rigor were labeled with a fluorescent ATP analog, 3′-DEAC-propylenediamine (p...

Full description

Saved in:
Bibliographic Details
Main Authors: Ibanez-Garcia, Delisa, Requejo-Isidro, Jose, Webb, Martin R., West, Timothy G., French, Paul, Ferenczi, Michael A.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2010
Subjects:
Muscle, Motility, and Motor Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3042588/
https://ncbi.nlm.nih.gov/pubmed/20923650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.07.052
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items