Response of Rigor Cross-bridges to Stretch Detected by Fluorescence Lifetime Imaging Microscopy of Myosin Essential Light Chain in Skeletal Muscle Fibers

We applied fluorescence lifetime imaging microscopy to map the microenvironment of the myosin essential light chain (ELC) in permeabilized skeletal muscle fibers. Four ELC mutants containing a single cysteine residue at different positions in the C-terminal half of the protein (ELC-127, ELC-142, ELC...

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Detalhes bibliográficos
Main Authors: Ushakov, Dmitry S., Caorsi, Valentina, Ibanez-Garcia, Delisa, Manning, Hugh B., Konitsiotis, Antonios D., West, Timothy G., Dunsby, Christopher, French, Paul M., Ferenczi, Michael A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2011
Assuntos:
Protein Structure and Folding
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3013043/
https://ncbi.nlm.nih.gov/pubmed/21056977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.149526
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