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Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins
The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported. The soluble Galdieria sulphuraria protein DCN1 was obtained in greater than 2 mg total synthesis yield per mL of reaction mixture from the Protemist XE, and the structure was...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3035758/ https://ncbi.nlm.nih.gov/pubmed/20637905 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.nbt.2010.07.003 |
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