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Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins

The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported. The soluble Galdieria sulphuraria protein DCN1 was obtained in greater than 2 mg total synthesis yield per mL of reaction mixture from the Protemist XE, and the structure was...

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Bibliografiska uppgifter
Huvudupphovsmän: Beebe, Emily T., Makino, Shin-ichi, Nozawa, Akira, Matsubara, Yuko, Frederick, Ronnie O., Primm, John G., Goren, Michael A., Fox, Brian G.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2010
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3035758/
https://ncbi.nlm.nih.gov/pubmed/20637905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.nbt.2010.07.003
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