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Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer

A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U pla...

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Detalhes bibliográficos
Main Authors: Pasternak, Anna, Hernandez, Frank J., Rasmussen, Lars M., Vester, Birte, Wengel, Jesper
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3035450/
https://ncbi.nlm.nih.gov/pubmed/20870750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq823
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