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A Magnetic Bead-Based Protein Kinase Assay with Dual Detection Techniques

A novel magnetic bead-based protein kinase assay was developed using MALDI-TOF mass spectrometry (MALDI-TOF MS) and immuno-chemifluorescence as two independent detection techniques. Abltide substrate was immobilized onto magnetic beads via non-covalent biotin-streptavidin interactions. This non-cova...

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Detalhes bibliográficos
Main Authors: Zhou, Guangchang, Sylvester, Juliesta E., Wu, Ding, Veach, Darren R., Kron, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3034244/
https://ncbi.nlm.nih.gov/pubmed/20807497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.08.034
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