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A Magnetic Bead-Based Protein Kinase Assay with Dual Detection Techniques
A novel magnetic bead-based protein kinase assay was developed using MALDI-TOF mass spectrometry (MALDI-TOF MS) and immuno-chemifluorescence as two independent detection techniques. Abltide substrate was immobilized onto magnetic beads via non-covalent biotin-streptavidin interactions. This non-cova...
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Autori principali: | , , , , |
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Natura: | Artigo |
Lingua: | Inglês |
Pubblicazione: |
2010
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Soggetti: | |
Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3034244/ https://ncbi.nlm.nih.gov/pubmed/20807497 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.08.034 |
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