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Photocleavable Peptide-Conjugated Magnetic Beads for Protein Kinase Assays by MALDI-TOF MS

Peptides were immobilized onto superparamagnetic beads via photocleavable linkers. This enabled simple, rapid, and label-free protein kinase assays via MALDI-TOF MS detection of substrate peptide phosphorylation. Abltide, a model substrate for the Abl protein tyrosine kinase model, was coupled onto...

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Detalhes bibliográficos
Publicado no:Bioconjug Chem
Main Authors: Zhou, Guangchang, Yan, Xiaoliang, Wu, Ding, Kron, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4562279/
https://ncbi.nlm.nih.gov/pubmed/20860375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc1003058
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