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Lesson from the Stoichiometry Determination of the Cohesin Complex: A Short Protease Mediated Elution Increases the Recovery from Cross-Linked Antibody-Conjugated Beads
[Image: see text] Affinity purification of proteins using antibodies coupled to beads and subsequent mass spectrometric analysis has become a standard technique for the identification of protein complexes. With the recent transfer of the isotope dilution mass spectrometry principle (IDMS) to the fie...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Chemical Society
2010
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3033704/ https://ncbi.nlm.nih.gov/pubmed/21043528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr100927x |
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