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Absolute Quantification and Stoichiometry Determination of Protein Complexes; A Novel and Cost-efficient Method to Produce Exact Equimolar Mixture of Standard Peptides

RP-125 Determination of protein complex stoichiometry can be achieved by absolute quantification of the interacting subunits based on isotope dilution mass spectrometry. Current available platforms for the generation of standard peptides are cost-intensive and deliver variable results concerning the...

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Bibliographische Detailangaben
Hauptverfasser: Pichler, P., Madalinski, M., Kurzbauer, R., Holzmann, J., Mechtler, K.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Association of Biomolecular Resource Facilities 2010
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2918182/
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