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Functional Compromises Among pH Tolerance, Site Specificity, and Sequence Tolerance for a DNA-Hydrolyzing Deoxyribozyme
We recently reported the identification by in vitro selection of 10MD5, a deoxyribozyme that requires both Mn(2+) and Zn(2+) to hydrolyze a single-stranded DNA substrate with formation of 5′-phosphate and 3′-hydroxyl termini. DNA cleavage by 10MD5 proceeds with k(obs) = 2.7 h(−1) and rate enhancemen...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3030634/ https://ncbi.nlm.nih.gov/pubmed/20923239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1013672 |
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