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Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine

To investigate the mechanism of trifluorothymidine (TFT)-induced DNA damage, we developed an enzymatic method for the synthesis of single-strand oligonucleotides containing TFT-monophosphate residues. Sixteen-mer oligonucleotides and 14-mer 5′-phosphorylated oligonucleotides were annealed to the tem...

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Autors principals: Suzuki, Norihiko, Fukushima, Masakazu
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3024846/
https://ncbi.nlm.nih.gov/pubmed/21128175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15257770.2010.535803
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