Purification of Heterotrimeric G Protein α Subunits by GST-Ric-8 Association: PRIMARY CHARACTERIZATION OF PURIFIED Gα(olf)

Ric-8A and Ric-8B are nonreceptor G protein guanine nucleotide exchange factors that collectively bind the four subfamilies of G protein α subunits. Co-expression of Gα subunits with Ric-8A or Ric-8B in HEK293 cells or insect cells greatly promoted Gα protein expression. We exploited these character...

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Detalhes bibliográficos
Principais autores: Chan, PuiYee, Gabay, Meital, Wright, Forrest A., Kan, Wei, Oner, Sukru S., Lanier, Stephen M., Smrcka, Alan V., Blumer, Joe B., Tall, Gregory G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2011
Assuntos:
Signal Transduction
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3024758/
https://ncbi.nlm.nih.gov/pubmed/21115479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.178897
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