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A Simple and Convenient G-quadruplex-Based Turn-On Fluorescence Assay for 3′ → 5′ Exonuclease Activity

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic label...

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Autors principals: Leung, Chung-Hang, Chan, Daniel Shiu-Hin, Man, Bradley Yat-Wah, Wang, Chuan-Jen, Lam, Wing, Cheng, Yung-Chi, Fong, Wang-Fun, Hsiao, Wendy Wen-Luan, Ma, Dik-Lung
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3021625/
https://ncbi.nlm.nih.gov/pubmed/21114271
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac1025896
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