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Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity

Elucidating how homing endonucleases undergo changes in recognition site specificity will facilitate efforts to engineer proteins for gene therapy applications. I-SceI is a monomeric homing endonuclease that recognizes and cleaves within an 18-base-pair (bp) target. It tolerates limited degeneracy i...

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Huvudupphovsmän: Joshi, Rakesh, Ho, Kwok Ki, Tenney, Kristen, Chen, Jui-Hui, Golden, Barbara L., Gimble, Frederick S.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2010
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3019082/
https://ncbi.nlm.nih.gov/pubmed/21029741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.10.029
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