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Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity
Elucidating how homing endonucleases undergo changes in recognition site specificity will facilitate efforts to engineer proteins for gene therapy applications. I-SceI is a monomeric homing endonuclease that recognizes and cleaves within an 18-base-pair (bp) target. It tolerates limited degeneracy i...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3019082/ https://ncbi.nlm.nih.gov/pubmed/21029741 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.10.029 |
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