Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and...

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Autors principals: Sander, Jeffry D., Dahlborg, Elizabeth J., Goodwin, Mathew J., Cade, Lindsay, Zhang, Feng, Cifuentes, Daniel, Curtin, Shaun J., Blackburn, Jessica S., Thibodeau-Beganny, Stacey, Qi, Yiping, Pierick, Christopher J., Hoffman, Ellen, Maeder, Morgan L., Khayter, Cyd, Reyon, Deepak, Dobbs, Drena, Langenau, David M., Stupar, Robert M., Giraldez, Antonio J., Voytas, Daniel F., Peterson, Randall T., Yeh, Jing-Ruey J., Joung, J. Keith
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3018472/
https://ncbi.nlm.nih.gov/pubmed/21151135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1542
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