Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Sander, Jeffry D., Dahlborg, Elizabeth J., Goodwin, Mathew J., Cade, Lindsay, Zhang, Feng, Cifuentes, Daniel, Curtin, Shaun J., Blackburn, Jessica S., Thibodeau-Beganny, Stacey, Qi, Yiping, Pierick, Christopher J., Hoffman, Ellen, Maeder, Morgan L., Khayter, Cyd, Reyon, Deepak, Dobbs, Drena, Langenau, David M., Stupar, Robert M., Giraldez, Antonio J., Voytas, Daniel F., Peterson, Randall T., Yeh, Jing-Ruey J., Joung, J. Keith
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3018472/
https://ncbi.nlm.nih.gov/pubmed/21151135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1542
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados