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Single-Cell Analysis of Phosphoinositide 3-Kinase (PI3K) and Phosphatase and Tensin Homolog (PTEN) Activation

A single-cell assay was developed to measure the activation of phosphoinositide 3-kinase (PI3K) using microanalytical chemical separations and a fluorescently labeled lipid substrate. Phosphatidyl-inositol 4,5 bisphosphate labeled on its acyl chain with Bodipy fluorescein (Bodipy Fl PIP(2)) was util...

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Autori principali: Jiang, Dechen, Sims, Christopher Eldridge, Allbritton, Nancy Lynn
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3017462/
https://ncbi.nlm.nih.gov/pubmed/21221426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/C005362G
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