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A protein-based EM label for RNA identifies the location of exons in spliceosomes
To locate key RNA features in the structure of the spliceosome by EM, we fused a sequence-specific RNA binding protein to a protein with a distinct donut-shaped structure. We used this fusion to label spliceosomes assembled on a pre-mRNA that contained the target sequence in the exons. The label is...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3004785/ https://ncbi.nlm.nih.gov/pubmed/18223660 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.1378 |
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